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The banana seed


The banana seed
Musa balbisiana seeds (photo by A. Vezina)
Musa balbisiana seeds (photo by A. Vezina)

The banana seed contains the embryonic plant that will develop into a seedling. The formation of the seed is part of the process of sexual reproduction, which involves the fertilization of an ovule (located inside the portion of the female flower called the gynoecium) by pollen produced by male flowers.

In dry mature seeds, the hard, leaky outer layer of the coat (the outer integument) acts like a cage, while the thin inner layer of the coat (the inner integument), which is lined with a cuticle,  is impermeable to water and provides an almost complete envelope surrounding the embryo and endosperm. Only wild species of bananas — and under certain circumstances cultivars that have residual fertility — produce seeds.

Seed structure

BananaSeedDiagram

The seed, like the one of the wild species, Musa balbisiana, has an extremely durable, wrinkled coat, the only opening of which is sealed by a shortened conical micropylar plug, also called an operculum (see figure). The seed coat consists of a thick hardened, but leaky, outer integument, and a thin inner integument, on the inner surface of which is a thick cuticle. Seeds of wild bananas are usually less than 1 cm in diameter[1].

The seed has two chambers: the larger one containing the endosperm and the embryo, and the smaller one the chalazal mass. Apart from a small discontinuity of parenchymatic tissue, the chambers are separated by the inner integument and cuticle. At the micropylar end, the inner integument projects into the micropylar canal[2], forming a cap to the larger chamber. In Musa acuminata, the larger chamber occupies just over 50% of seed volume, while the smaller chamber, the micropylar plug and other outer integument material account for the remaining volume[3][4].

While size, colour and shape varied, a study of 20 species of wild and edible bananas found this basic seed structure and anatomy in all of them[5].

The water content of fresh banana seeds ranges from 31 to 46%, falling to below 10% when seeds are dried at around 20oC[5]. The chalazal mass, which in fresh seeds was observed to be "full of water", shrivels upon drying[5].

Germination

The basic external requirements for germination are water, oxygen and an appropriate temperature. The germination of a seed begins with water uptake (imbibition), followed by embryo expansion along its axis through cell elongation, and ends with the breaking-through of the seed coat by the embryo root (radicle), then shoot (plumule). In the banana seed, the endosperm disappears within two weeks and within three weeks, the seedling has a lateral root system, and a prominent shoot[6], in which the first leaves have elongated beyond the cotyledonary sheath[7]. By the fourth week, the seedling is dependent on the root system for inorganic nutrition[6].

Imbibition

Water uptake, measured as increase in weight, by intact sand-papered and pierced Musa acuminata seeds. The seed coat was pierced adjacent to the chalazal mass. Sand-papered seeds were abraded between the micropylar plug and chalazal mass. (Data from Wattananchaiyingcharoen 1990)
Water uptake, measured as increase in weight, by intact sand-papered and pierced Musa acuminata seeds. The seed coat was pierced adjacent to the chalazal mass. Sand-papered seeds were abraded between the micropylar plug and chalazal mass. (Data from Wattananchaiyingcharoen 1990)

Water must enter the chamber containing the embryo and endosperm before germination can occur. Experiments have shown that the seed's outer integument is leaky since rupturing the outer integument of Musa acuminata seeds by sandpapering did not increase the absorption of water.   During imbibition, the weight of the sandpapered seeds increased by the same proportion (33%) as the one of intact seeds (see graph)[8].

On the other hand, piercing with a needle the outer and inner integument (with its cuticle) led to a more rapid absorption of water and an additional 7% increase in weight [8], consistent with the inner integument being a barrier to water movement into the chamber containing the embryo and endosperm.

Where, and under what circumstances, water penetrates the inner integument is unknown. In many non-banana species it happens via water gaps[9]. In bananas, water gaps might develop around the micropylar plug[10], or in the area of the chalazal mass, where the inner integument is discontinuous, and parenchymatic tissue separates the outer and inner chambers[2].

The structure of the banana seed with its leaky outer integument means that the seed imbibes but the embryo and endosperm might not absorb water because of the impermeable inner integument and non functional water gaps.

While seeds will not germinate without imbibing water, imbibition does not necessarily lead to germination. Non-viable seeds, for one, will not germinate. Seed viability in a narrow sense refers to viability of the embryo, but it can be extended to include absence of the embryo or the endosperm, or absence of a functional connection between the embryo and endosperm.

Dormancy

A seed has one living component, the embryo. To germinate, not only does the embryo have to come into contact with water, the seed also has to be non-dormant.

Opinions on dormancy in banana seeds differ. The embryo of Musa balbisiana, for one, has been said not to have dormancy[11] because it is easy to culture embryos excised from fresh seeds[12]. Usually, more than 90% of these excised embryos of Musa balbisiana grow into plantlets[13]. On the other hand, only 68 to 75% of embryos excised from fresh seeds of Musa acuminata developed into plantlets[13]; whereas in studies using seeds from the products of open-pollinated wild bananas and interspecific crosses[13], dried seeds of Musa acuminata[14], and crosses between East African highland banana cultivars and Calcutta 4[15], the success rate of embryo culture was well below 50%. These observations suggest that, in edible bananas and some wild banana species, dormancy may have a role in preventing embryo growth into plantlets.

It is likely that there are different types of dormancy among Musa species, with contributions from the seed coat and the embryo. The necessary experimentation to clearly define the roles of the coat, embryo and other tissues in dormancy have not been done, but that banana seeds can become dormant appears beyond doubt[16].

Cues for germination

In their natural habitat, humid tropical forests, banana seeds may lie viable for years, often germinating at the same time and in large numbers when soil is disturbed or vegetation removed[17]. The higher temperature of exposed soil in gaps in the forest suggests that temperature is a cue for banana seeds to germinate. Under greenhouse conditions, the germination of Musa balbisiana seeds was greatest when temperature fluctuations were large[18].

It is not known, however, how seeds sense temperatures that lead to germination. Work on Arabidopsis thaliana showed that increasing gibberellin (GA) content and decreasing abscisic acid (ABA) content in the seed promoted germination, and vice versa, and that interactions between ABA and GA played key roles in regulating germination at different temperatures[19]. Incubating intact fresh hybrid AA diploid seeds (Pisang Jajee x Musa acuminata ssp. burmannicoides) in GA for several days before embryo culture resulted in an increase in embryo germination rate in vitro[20], although the mechanism is unclear.

Growth of the embryo

The first visual evidence of germination is the expulsion of the micropylar plug from the micropylar canal. The plug is forced out cleanly along a line of "predetermined breakage" (the abscission layer)[6][1] by pressure exerted by the axial expansion of the embryo, perhaps assisted, at least in fresh seeds, by pressure resulting from the absorption of water from its environment by the chalazal mass.

In laboratory-based studies, the exudation of a drop of brownish fluid from the micropyle has been observed to precede the expulsion of the micropylar plug[6][8]. This drop may be the consequence of a build-up of hydrostatic pressure in the chamber containing the embryo and the endosperm as the embryo begins to expand, forcing fluid in the seed cavity through breakage points around the micropylar plug. This is loosely analogous to guttation in non-transpiring leaves in that hydrostatic pressure is the driving force for the exudation.

Studies using ruptured seed coats show that the expanding embryo emerges through the rupture[18][8], the path of least resistance, suggesting that some force is needed to dislodge the micropylar plug[16]. In nature, the need to exert a force might select against embryos that would otherwise grow into seedlings that are "less fit"[16]. If this is the case, bypassing this step by excising embryos and culturing them will include any such less fit seedlings[16].

Seeds of cultivated bananas

When edible bananas that have residual fertility are used in breeding, the number of seeds produced is generally low to very low. Both seed viability and seed dormancy can impact on germination rates. In a study of the products of crosses between 20 East African highland banana cultivars and Calcutta 4, the number of seeds per bunch ranged from 3 to 26, with an average of only 9 seeds per bunch. Of these seeds, only 42% had embryos, of which only 9% germinated following embryo extraction and germination in vitro[15]. Whether this low rate of germination reflects low embryo viability per se or embryo dormancy is not known.

Identifying viable seeds has also proved difficult. In the same study, the presence of a hard outer integument (hard black seeds) was used as a predictor of viability. Whereas 72% of the small number of seeds harvested from the 20 pollinated East African highland banana cultivars met this criterion, only 59% had an embryo[15]. As such, less than half of the harvested seeds were potentially viable.

References

1. Graven, P., De Koster, C. G., Boon, J. J., Bouman, F. 1996. Structure and macromolecular composition of the seed coat of the Musaceae. Annals of Botany 77:105-122.
2. McGahan, M. W. 1961. Studies on the seed of banana. I. Anatomy of the seed and embryo of Musa balbisiana. American Journal of Botany 48:230-238.
3. Calculated by Jane Gibbs and David Turner using data in Vineesh et al. (2015) and Wattanachaiyingcharoen (1989).
4. Wattanachaiyingcharoen D (1989) Improving seed germination and breaking seed dormancy of banana seed (Musa acuminata subsp.) MSc Prelim Report. The University of Western Australia.
5. Chin, H. F. 1996. Germination and storage of banana seeds. In: New Frontiers in resistance breeding for nematode, Fusarium and Sigatoka. Frison, E. A,, Horry, J.P. and De Waele, D. (Eds.) Proc. Workshop held in Kuala Lumpur, Malaysia, 2-5 October 1995, 218-227. INIBAP, Los Banos, Phillipines.
6. Simmonds, N. W. 1959. Experiments on the germination of banana seeds. Tropical Agriculture (Trinidad) 36(4):259-273.
7. McGahan, M. W. 1961. Studies on the seed of banana. II. The anatomy and morphology of the seedling of Musa balbisiana. American Journal of Botany 48:630-637.
8. Wattanachaiyingcharoen D (1990) Viability, germination and dormancy of banana seed (Musa acuminata sub sp.) MSc thesis. The University of Western Australia.
9. Gama-Arachchige, N. S., Baskin, J. M., Geneve, R.L., Baskin, C. C. 2013. Identification and characterization of ten new water gaps in seeds and fruits with physical dormancy and classification of water-gap complexes. Annals of Botany 112(1):69-84.
10. Puteh, A. B., Aris, E. M., Sinniah, U. R., Rahman, M. M., Mohamad, R. B., Abdullah, N. A. P. 2011. Seed anatomy, moisture content and scarification influence on imbibition in wild banana (Musa acuminata Colla) ecotypes. African Journal of Biotechnology 10:14373-14379.
11. Stotzky, G., Cox, E.A. and Goos, R.D. 1962. Seed Germination Studies in Musa. I. Scarification and Aseptic Germination of Musa balbisiana. American Journal of Botany 49(5):515-520.
12. Cox, E., Stotzky, G., Goos, R. D. 1960. In vitro culture of Musa balbisiana Colla embryos. Nature 185:403-404.
13. Bakry, F. 2008. Zygotic embryo rescue in bananas. Fruits 63(2):111-115.
14. Vineesh, P. S., Skaria, R., Mukunthakumar, S., Padmesh, P., Decruse, S. W. 2015. Seed germination and cryostorage of Musa acuminata subsp. burmannica from Western Ghats. South African Journal of Botany 100:158-163.
15. Ssebuliba, R., Talengera, D., Makumbi, D., Namanya, P., Tenkouana, A., Tushemereirwe, W., Pillay, M. 2006. Reproductive efficiency and breeding potential of East African highland (Musa AAA-EA) bananas. Field Crops Research 95(2-3):250-255.
16. Fortescue, J. A., Turner, D. W. 2011. Reproductive Biology pp. 145-179 In: Pillay, M., Tenkouano, A. (Eds.) Banana Breeding: Progress and Challenges. CRC Press, Boca Raton.
17. Simmonds, N. W. 1962. The Evolution of the Bananas. Longmans, London, UK.
18. Stotzky, G., Cox, E. A. 1962. Seed germination studies in Musa. II. Alternating temperature requirement for the germination of Musa balbisiana. American Journal of Botany 49(7):763-770.
20. Arun, K., Uma, S., Saraswathi, M. S., Backiyarani, S., Durai, P. 2013. Effects of whole seed priming on the in vitro germination of hybrid banana embryos (Musa spp.). Seed Science and Technology 41(3):439-451.