ISSN 1729-6196

Banana and plantain embryogenic cell suspensions - Technical Guidelines 8 (1.3 Mb)
Hannelore Strosse, Régis Domergue, Bart Panis, Jean-Vincent Escalant and François Côte, 2003
These guidelines present two protocols to produce embryogenic cell suspensions by using scalps or immature male flowers. The protocols have been developed by the Laboratory of Tropical Crop Improvement of the Katholieke Universiteit Leuven (KULeuven) and the cellular biology laboratory of the Centre de coopération internationale en recherche agronomique pour le développement (CIRAD).

ISSN 1729-6196

Global evaluation of Musa germplasm for resistance to Fusarium wilt, Mycosphaerella leaf spot diseases, and nematodes: Performance evaluation - Technical Guidelines 7 (1.1 Mb)
Jean Carlier, Dirk de Waele and Jean-Vincent Escalant, 2003
These Technical Guidelines replace the Technical Guidelines 1 (Screening of Musa (germplasm for resistance and tolerance to nematodes) and 3 (Evaluation of Musa germplasm for resistance to Sigatoka diseases and Fusarium wilt) as far as performance evaluations are concerned.
Technical Guidelines for in-depth evaluations, which were also part of Technical Guidelines 3, are published separately. The Fusarium wilt and Mycosphaerella leaf spot sections have been updated following recommendations and comments made after analysing IMTP II results.

		ISSN 1729-6196

Global evaluation of Musa germplasm for resistance to Fusarium wilt, Mycosphaerella leaf spot diseases, and nematodes: In-depth evaluation - Technical Guidelines 6 (891 Kb)
Jean Carlier, Dirk de Waele and Jean-Vincent Escalant, 2002
These Technical Guidelines replace Technical Guidelines 1 (Screening of Musa Germplasm for resistance and tolerance to nematodes) and 3 (Evaluation of Musa germplasm for resistance to Sigatoka diseases and Fusarium wilt) as far as indepth evaluations are concerned. Technical Guidelines for performance evaluations are published separately. These changes were made to reflect the apparition of a third disease caused by a Mycosphaerella fungus (eumusae leaf spot disease), to simplify the guidelines, and to group under one cover the evaluations for the main pests and diseases affecting bananas.

ISSN 1729-6196

Cryopreservation of Musa germplasm - Technical Guidelines 5 (961 Kb)
Bart Panis and Nguyen Tien Thinh, 2001
This publication describes cryopreservation methods developed for Musa tissue at the Katholieke Universiteit Leuven in Belgium and the Japanese International Research Centre for Agricultural Sciences. These methodologies are specific to the type of banana tissue to be cryopreserved: individual meristems, meristem clumps (cauliflower-like structures), embryogenic cell suspensions and zygotic embryos.

	ISSN 1729-6196

Evaluation of Musa germplasm for resistance to Sigatoka diseases and Fusarium wilt - Technical Guidelines 3 (1.18 Mb)
Gisella Orjeda, 1998
Contains protocols for the evaluation of resistance to leaf spot and wilt diseases. Two protocols were defined to enable two levels of evaluation. The protocols were developed in response to increasing demand from numerous national programmes that wished to evaluate germplasm under local conditions and because of the need to perform in-depth studies on the pathogens and their hosts at a more limited number of locations.