Proteome Analysis for in vitro cultured banana plantlets

Posted by asiimwefx on 23 Sep 2012 14:24

I am studying the proteome, protein profile (proline, superoxidase dismutase, glycinebetaine, asparagine and abscisic acid) and antioxidant enzyme activity (peroxidase, catalase, glutathione reductase, ascorbate peroxidase and superoxide dismutase) of in vitro cultured banana plantlets to sorbitol induced drought stress. What is the simplest protocol for analysing changes in the proteome and enzyme activity as a result of the drought stress simulation?

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Posted by sebastienc on 23 Sep 2012 20:57

Dear Francis,

Analyzing the protein activity is a different protocol than quantifying the different proteins at once. For testing the antioxidant enzyme activity I believe several commercial kits are available. For quantifyging the proteome, I would like to refer to some publications of us:

Carpentier, S. C., Coemans, B., Podevin, N., Laukens, K., et al., Functional genomics in a non?model
crop: transcriptomics or proteomics? Physiol. Plant. 2008, 133, 117?130.

Carpentier, S. C., Panis, B., Vertommen, A., Swennen, R., et al., Proteome analysis of non?model
plants: A challenging but powerful approach. Mass Spec. Rev. 2008, 27, 354?377.

Vanhove, A.?C., Vermaelen, W., Panis, B., Swennen, R., Carpentier, S., Screening the banana
biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to
discover tolerant varieties and understand homeostasis? Frontiers in Plant Science 2012

Carpentier, S. C., Witters, E., Laukens, K., Deckers, P., et al., Preparation of protein extracts from
recalcitrant plant tissues: An evaluation of different methods for two?dimensional gel electrophoresis
analysis. Proteomics 2005, 5, 2497?2507.

Carpentier, S. C., Swennen, R., Panis, B., in: Walker, J. M. (Ed.), The protein protocols handbook,
Humana Press, Totowa 2009, pp. 107?117

sebastien carpentier

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